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The Effects of (99mTc) Hm-Pao Labeling on Lymphocyte Functions

10.4274/mirt.24.01.11

  • Özden Ülker
  • Sermin Genç
  • Halil Ateş
  • Neşe Atabey
  • Hatice Durak

Mol Imaging Radionucl Ther 2015;24(1):43-43

Results:

In youth who changed their weight status, there were significant associations (p<0.05) between changes in BMI percentile and changes in leptin, insulin and cortisol (partial R[sup]2[/sup]= 0.35, 0.13 and 0.11, respectively), after accounting for race, sex and changes in pubertal status and aerobic power. Our key findings were that youth who became overweight (NO) showed greater changes for leptin (+205% vs. -21%) and cortisol (-33% vs. +13%), p<0.05 than those who reverted from overweight to normal weight (ON).

Conclusion:

Natural changes in weight status in youth show a relationship with changes in leptin, insulin and cortisol levels and the hormonal changes appear to be more sensitive to increases, rather than reductions, in weight status.

Methods:

In this study, we evaluated the effects of (99mTc) HM-PAO labeling on lymphocyte adhesion, proliferation, migration and apoptosis. We used NC-NC lymphoblastoid cell line as the lymphocyte population. (99mTc) HM-PAO labeling decreased cell adhesion, proliferation and motility whereas induced apoptosis, and cell cycle arrest. Proliferation assays were performed both using MTT and ELISA tests with 24 hours intervals following labeling.

Results:

It was recorded that the rate of decrease in proliferation was up to 70% by the 4th day after labeling. (99mTc) HM-PAO labeling led a 35% decrease on adhesion ability of the cells on fibronectin. By using Boyden chamber motility assay, we showed that both spontaneous and MCP-1 induced lymphocyte motility were potently blocked by (99mTc) HM-PAO labeling. The rate of decrease in motility was approximately five times. In addition, we observed a 12 times increase in the apoptosis rate within the (99mTc) HM-PAO treated cells compared to the control cells. Besides it was observed that cell cycle arrest was induced starting from 3rd day after (99mTc) HM-PAO treatment.

Conclusion:

Based on our data (99mTc) HM-PAO labeling has damaging effects on lymphocyte functions including cell adhesion, proliferation, motility and viability in in vitro.

Keywords: 99mTc HM-PAO,apoptosis,migration,adhesion